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atac seq library preparation kit  (Illumina Inc)


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    Illumina Inc atac seq library preparation kit
    Stable mobilization of CEBPs and PPARG upon transient TAK-981 treatment in pre-adipocytes. ( A <t>)</t> <t>ATAC-seq</t> experimental layout in hTERT A41hWAT-SVF pre-adipocytes. Time series analysis and hierarchical clustering of significant variations in chromatin accessibility at day 0 (D0), 12 h (12h), and day (D7) after TAK-981 treatment. ( C ) GOBP analysis of ATAC-seq clusters 2 and 6 revealed in panel (B). See and for an analysis of all clusters. ( D – F ) Volcano plots displaying the results of ATAC-seq inferred differential TF activity analyses performed at D0, 12h, and D7 after TAK-981 or DMSO treatment. Colored dots indicate significant differentially mobilized TFs (TAK-981 versus DMSO). Differential binding score >0.05 and pAdj <0.001. ( G ) Time series analysis inferred TF activity over time (TAK-981 versus DMSO) at D0, 12h, and D7 after TAK-981 treatment. ( H ) Western blot analysis of CEBPB SUMOylation in DMSO and TAK-981-treated cells. ( I ) Western blot analysis of CEBPB and PPARG in DMSO, rosiglitazone, TAK-981-treated cells and cotreated hTERT A41hWAT-SVF pre-adipocytes. ( J ) Western blot analysis of PPARG in DMSO, rosiglitazone, TAK-981-treated cells and cotreated hASCs.
    Atac Seq Library Preparation Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 985 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tagmentation/Illumina+Tagment+DNA+Enzyme+and+Buffer+Small+Kit/pmc13000455-115-18-17
    Average 97 stars, based on 985 article reviews
    atac seq library preparation kit - by Bioz Stars, 2026-10
    97/100 stars

    Images

    1) Product Images from "Transient SUMOylation inhibition in human pre-adipocytes stably imprints a transcriptional beiging fate"

    Article Title: Transient SUMOylation inhibition in human pre-adipocytes stably imprints a transcriptional beiging fate

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkag232

    Stable mobilization of CEBPs and PPARG upon transient TAK-981 treatment in pre-adipocytes. ( A ) ATAC-seq experimental layout in hTERT A41hWAT-SVF pre-adipocytes. Time series analysis and hierarchical clustering of significant variations in chromatin accessibility at day 0 (D0), 12 h (12h), and day (D7) after TAK-981 treatment. ( C ) GOBP analysis of ATAC-seq clusters 2 and 6 revealed in panel (B). See and for an analysis of all clusters. ( D – F ) Volcano plots displaying the results of ATAC-seq inferred differential TF activity analyses performed at D0, 12h, and D7 after TAK-981 or DMSO treatment. Colored dots indicate significant differentially mobilized TFs (TAK-981 versus DMSO). Differential binding score >0.05 and pAdj <0.001. ( G ) Time series analysis inferred TF activity over time (TAK-981 versus DMSO) at D0, 12h, and D7 after TAK-981 treatment. ( H ) Western blot analysis of CEBPB SUMOylation in DMSO and TAK-981-treated cells. ( I ) Western blot analysis of CEBPB and PPARG in DMSO, rosiglitazone, TAK-981-treated cells and cotreated hTERT A41hWAT-SVF pre-adipocytes. ( J ) Western blot analysis of PPARG in DMSO, rosiglitazone, TAK-981-treated cells and cotreated hASCs.
    Figure Legend Snippet: Stable mobilization of CEBPs and PPARG upon transient TAK-981 treatment in pre-adipocytes. ( A ) ATAC-seq experimental layout in hTERT A41hWAT-SVF pre-adipocytes. Time series analysis and hierarchical clustering of significant variations in chromatin accessibility at day 0 (D0), 12 h (12h), and day (D7) after TAK-981 treatment. ( C ) GOBP analysis of ATAC-seq clusters 2 and 6 revealed in panel (B). See and for an analysis of all clusters. ( D – F ) Volcano plots displaying the results of ATAC-seq inferred differential TF activity analyses performed at D0, 12h, and D7 after TAK-981 or DMSO treatment. Colored dots indicate significant differentially mobilized TFs (TAK-981 versus DMSO). Differential binding score >0.05 and pAdj <0.001. ( G ) Time series analysis inferred TF activity over time (TAK-981 versus DMSO) at D0, 12h, and D7 after TAK-981 treatment. ( H ) Western blot analysis of CEBPB SUMOylation in DMSO and TAK-981-treated cells. ( I ) Western blot analysis of CEBPB and PPARG in DMSO, rosiglitazone, TAK-981-treated cells and cotreated hTERT A41hWAT-SVF pre-adipocytes. ( J ) Western blot analysis of PPARG in DMSO, rosiglitazone, TAK-981-treated cells and cotreated hASCs.

    Techniques Used: Activity Assay, Binding Assay, Western Blot

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    Construct:

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    Next-Generation Sequencing:

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    Polymerase Chain Reaction:

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    Formalin-fixed Paraffin-Embedded:

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    Stable mobilization of CEBPs and PPARG upon transient TAK-981 treatment in pre-adipocytes. ( A <t>)</t> <t>ATAC-seq</t> experimental layout in hTERT A41hWAT-SVF pre-adipocytes. Time series analysis and hierarchical clustering of significant variations in chromatin accessibility at day 0 (D0), 12 h (12h), and day (D7) after TAK-981 treatment. ( C ) GOBP analysis of ATAC-seq clusters 2 and 6 revealed in panel (B). See and for an analysis of all clusters. ( D – F ) Volcano plots displaying the results of ATAC-seq inferred differential TF activity analyses performed at D0, 12h, and D7 after TAK-981 or DMSO treatment. Colored dots indicate significant differentially mobilized TFs (TAK-981 versus DMSO). Differential binding score >0.05 and pAdj <0.001. ( G ) Time series analysis inferred TF activity over time (TAK-981 versus DMSO) at D0, 12h, and D7 after TAK-981 treatment. ( H ) Western blot analysis of CEBPB SUMOylation in DMSO and TAK-981-treated cells. ( I ) Western blot analysis of CEBPB and PPARG in DMSO, rosiglitazone, TAK-981-treated cells and cotreated hTERT A41hWAT-SVF pre-adipocytes. ( J ) Western blot analysis of PPARG in DMSO, rosiglitazone, TAK-981-treated cells and cotreated hASCs.
    Tagment Tn5 Transposase, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tagmentation/Illumina+Tagment+DNA+Enzyme+and+Buffer+Small+Kit/pmc13000455-121-26-32
    Average 97 stars, based on 1 article reviews
    tagment tn5 transposase - by Bioz Stars, 2026-10
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    td enzyme  (Illumina Inc)
    97
    Illumina Inc td enzyme
    Stable mobilization of CEBPs and PPARG upon transient TAK-981 treatment in pre-adipocytes. ( A <t>)</t> <t>ATAC-seq</t> experimental layout in hTERT A41hWAT-SVF pre-adipocytes. Time series analysis and hierarchical clustering of significant variations in chromatin accessibility at day 0 (D0), 12 h (12h), and day (D7) after TAK-981 treatment. ( C ) GOBP analysis of ATAC-seq clusters 2 and 6 revealed in panel (B). See and for an analysis of all clusters. ( D – F ) Volcano plots displaying the results of ATAC-seq inferred differential TF activity analyses performed at D0, 12h, and D7 after TAK-981 or DMSO treatment. Colored dots indicate significant differentially mobilized TFs (TAK-981 versus DMSO). Differential binding score >0.05 and pAdj <0.001. ( G ) Time series analysis inferred TF activity over time (TAK-981 versus DMSO) at D0, 12h, and D7 after TAK-981 treatment. ( H ) Western blot analysis of CEBPB SUMOylation in DMSO and TAK-981-treated cells. ( I ) Western blot analysis of CEBPB and PPARG in DMSO, rosiglitazone, TAK-981-treated cells and cotreated hTERT A41hWAT-SVF pre-adipocytes. ( J ) Western blot analysis of PPARG in DMSO, rosiglitazone, TAK-981-treated cells and cotreated hASCs.
    Td Enzyme, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tagmentation/Illumina+Tagment+DNA+Enzyme+and+Buffer+Small+Kit/pmc12915527-164-26-29
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    tn5 tagmented lambda dna  (New England Biolabs)
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    New England Biolabs tn5 tagmented lambda dna
    Stable mobilization of CEBPs and PPARG upon transient TAK-981 treatment in pre-adipocytes. ( A <t>)</t> <t>ATAC-seq</t> experimental layout in hTERT A41hWAT-SVF pre-adipocytes. Time series analysis and hierarchical clustering of significant variations in chromatin accessibility at day 0 (D0), 12 h (12h), and day (D7) after TAK-981 treatment. ( C ) GOBP analysis of ATAC-seq clusters 2 and 6 revealed in panel (B). See and for an analysis of all clusters. ( D – F ) Volcano plots displaying the results of ATAC-seq inferred differential TF activity analyses performed at D0, 12h, and D7 after TAK-981 or DMSO treatment. Colored dots indicate significant differentially mobilized TFs (TAK-981 versus DMSO). Differential binding score >0.05 and pAdj <0.001. ( G ) Time series analysis inferred TF activity over time (TAK-981 versus DMSO) at D0, 12h, and D7 after TAK-981 treatment. ( H ) Western blot analysis of CEBPB SUMOylation in DMSO and TAK-981-treated cells. ( I ) Western blot analysis of CEBPB and PPARG in DMSO, rosiglitazone, TAK-981-treated cells and cotreated hTERT A41hWAT-SVF pre-adipocytes. ( J ) Western blot analysis of PPARG in DMSO, rosiglitazone, TAK-981-treated cells and cotreated hASCs.
    Tn5 Tagmented Lambda Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tagmentation/Lambda+DNA/pm41886469-392-16-19
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    tn5 tagmented lambda dna - by Bioz Stars, 2026-10
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    e7770l illumina tagment dna enzyme  (New England Biolabs)
    99
    New England Biolabs e7770l illumina tagment dna enzyme
    Stable mobilization of CEBPs and PPARG upon transient TAK-981 treatment in pre-adipocytes. ( A <t>)</t> <t>ATAC-seq</t> experimental layout in hTERT A41hWAT-SVF pre-adipocytes. Time series analysis and hierarchical clustering of significant variations in chromatin accessibility at day 0 (D0), 12 h (12h), and day (D7) after TAK-981 treatment. ( C ) GOBP analysis of ATAC-seq clusters 2 and 6 revealed in panel (B). See and for an analysis of all clusters. ( D – F ) Volcano plots displaying the results of ATAC-seq inferred differential TF activity analyses performed at D0, 12h, and D7 after TAK-981 or DMSO treatment. Colored dots indicate significant differentially mobilized TFs (TAK-981 versus DMSO). Differential binding score >0.05 and pAdj <0.001. ( G ) Time series analysis inferred TF activity over time (TAK-981 versus DMSO) at D0, 12h, and D7 after TAK-981 treatment. ( H ) Western blot analysis of CEBPB SUMOylation in DMSO and TAK-981-treated cells. ( I ) Western blot analysis of CEBPB and PPARG in DMSO, rosiglitazone, TAK-981-treated cells and cotreated hTERT A41hWAT-SVF pre-adipocytes. ( J ) Western blot analysis of PPARG in DMSO, rosiglitazone, TAK-981-treated cells and cotreated hASCs.
    E7770l Illumina Tagment Dna Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tagmentation/NEBNext+Ultra+II+RNA+Library+Prep+Kit/pm41916318-278-160-156
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    Image Search Results


    (A) Browser snapshot of CUT&Tag profiling in K562 cells for the H3K27me3, H3K27me2, H3K27me1, and H3K27ac modifications, and RNAPIIS2/5p at the POU5F1 (OCT4) locus. A Polycomb domain overlaps the PSOR1S1C gene. (B) (top) Pearson correlation heatmap of active RNAPII versus H3K27 modifications at coding genes including a 1 kilobase promoter region upstream (n=19,946). (bottom) Signal heatmap of correlated gene values after log2 transformation. Genes in annotated Polycomb domains are highlighted in red and show high H3K27me3, low active RNAPII signal. (C) Gating schema based on DRAQ5 measurements of DNA-content used to isolate S-phase fractions via fluorescence-activated cell sorting (FACS). (D) CUT&Tag signal at genes in Polycomb domains (n=2,861) ranked by replication timing (y-axis) over the S-phase fractions (x-axis) from (C). Signal is shown as fraction of the maximum averaged across two replicates. Log10(max RPKM) per gene is shown in purple for the H3K27me1, H3K27me2, and H3K27me3 modifications to demonstrate high H3K27me3 signal and relatively less H3K27me2 and H3K27me1 signal at these genes.

    Journal: bioRxiv

    Article Title: Histone H3K27 methylation states are sequentially catalyzed in cycling cells

    doi: 10.64898/2026.04.30.721988

    Figure Lengend Snippet: (A) Browser snapshot of CUT&Tag profiling in K562 cells for the H3K27me3, H3K27me2, H3K27me1, and H3K27ac modifications, and RNAPIIS2/5p at the POU5F1 (OCT4) locus. A Polycomb domain overlaps the PSOR1S1C gene. (B) (top) Pearson correlation heatmap of active RNAPII versus H3K27 modifications at coding genes including a 1 kilobase promoter region upstream (n=19,946). (bottom) Signal heatmap of correlated gene values after log2 transformation. Genes in annotated Polycomb domains are highlighted in red and show high H3K27me3, low active RNAPII signal. (C) Gating schema based on DRAQ5 measurements of DNA-content used to isolate S-phase fractions via fluorescence-activated cell sorting (FACS). (D) CUT&Tag signal at genes in Polycomb domains (n=2,861) ranked by replication timing (y-axis) over the S-phase fractions (x-axis) from (C). Signal is shown as fraction of the maximum averaged across two replicates. Log10(max RPKM) per gene is shown in purple for the H3K27me1, H3K27me2, and H3K27me3 modifications to demonstrate high H3K27me3 signal and relatively less H3K27me2 and H3K27me1 signal at these genes.

    Article Snippet: Confirmation of H3K27 methylation profiling with designer barcoded nucleosomes: (A) Cleavage Under Targets & Tagmentation (CUT&Tag) chromatin profiling paradigm with a synthetic nucleosome spike-in panel (EpiCypher, Cat# 19-1002).

    Techniques: Transformation Assay, Fluorescence, FACS

    (A) Experimental design for 8-hour treatment of cycling K562 cells with small-molecule inhibitors of PRC2. (B) CUT&Tag signal at genes in Polycomb domains (n=2,861) ranked by replication timing (y-axis) over the S-phase fractions (x-axis). Signal is shown as fraction of the maximum averaged across two replicates. Max RPKM is computed per treatment across the S-phase time course. Log10(max RPKM) per gene is show in purple for active RNAPII, H3K27ac, H3K27me1, H3K27me2, and H3K27me3 (left to right). The heatmaps show signal after treatment for 8 hours with DMSO (vehicle control, top), the EZH2 inhibitor EPZ-6438 (middle), and the EED inhibitor EED-226 (bottom). (C) Statistical paradigm for testing the relationship between drug effects versus replication timing at genes in Polycomb domains. Scatter plots are from Supplementary Figure 8. Genes above an absolute of log2 fold-change of 0.2 are colored red for up in treatment or blue for down in treatment, or grey for Log2 fold-change < 0.2. (D) Linear regression analysis (y-axis) of response to EPZ-6438 (top), and EED-226 (bottom) within S-phase fractions (x-axis) versus replication timing at genes in Polycomb domains. Stars indicate a significance < 0.001 after FDR correction. Bar color is Spearman’s rho (E) PRC2 inhibition at early replicating genes in and outside Polycomb domains. Median log2 fold-change for EPZ-6438 (top) and EED-226 (bottom) versus DMSO is shown for H3K27me2 (y-axis) and H3K27ac (x-axis) in the S2 fraction. The fold difference between Log2 fold-change in the S1 versus S2 fraction is shown for differences greater than ±0.2. Stars indicate a significant difference in Log2 fold-change values by the Wilcoxon test with a p-value < 0.001 after Bonferroni correction.

    Journal: bioRxiv

    Article Title: Histone H3K27 methylation states are sequentially catalyzed in cycling cells

    doi: 10.64898/2026.04.30.721988

    Figure Lengend Snippet: (A) Experimental design for 8-hour treatment of cycling K562 cells with small-molecule inhibitors of PRC2. (B) CUT&Tag signal at genes in Polycomb domains (n=2,861) ranked by replication timing (y-axis) over the S-phase fractions (x-axis). Signal is shown as fraction of the maximum averaged across two replicates. Max RPKM is computed per treatment across the S-phase time course. Log10(max RPKM) per gene is show in purple for active RNAPII, H3K27ac, H3K27me1, H3K27me2, and H3K27me3 (left to right). The heatmaps show signal after treatment for 8 hours with DMSO (vehicle control, top), the EZH2 inhibitor EPZ-6438 (middle), and the EED inhibitor EED-226 (bottom). (C) Statistical paradigm for testing the relationship between drug effects versus replication timing at genes in Polycomb domains. Scatter plots are from Supplementary Figure 8. Genes above an absolute of log2 fold-change of 0.2 are colored red for up in treatment or blue for down in treatment, or grey for Log2 fold-change < 0.2. (D) Linear regression analysis (y-axis) of response to EPZ-6438 (top), and EED-226 (bottom) within S-phase fractions (x-axis) versus replication timing at genes in Polycomb domains. Stars indicate a significance < 0.001 after FDR correction. Bar color is Spearman’s rho (E) PRC2 inhibition at early replicating genes in and outside Polycomb domains. Median log2 fold-change for EPZ-6438 (top) and EED-226 (bottom) versus DMSO is shown for H3K27me2 (y-axis) and H3K27ac (x-axis) in the S2 fraction. The fold difference between Log2 fold-change in the S1 versus S2 fraction is shown for differences greater than ±0.2. Stars indicate a significant difference in Log2 fold-change values by the Wilcoxon test with a p-value < 0.001 after Bonferroni correction.

    Article Snippet: Confirmation of H3K27 methylation profiling with designer barcoded nucleosomes: (A) Cleavage Under Targets & Tagmentation (CUT&Tag) chromatin profiling paradigm with a synthetic nucleosome spike-in panel (EpiCypher, Cat# 19-1002).

    Techniques: Control, Inhibition

    (A) CUT&Tag signal at genes outside Polycomb domains enriched for H3K27me2 (top, n=2,268) and (B) H3K27me1 (bottom, n=3,872) ranked by replication timing (y-axis) over the S-phase fractions (x-axis). Signal is shown as fraction of the maximum (dilution) averaged across two replicates.

    Journal: bioRxiv

    Article Title: Histone H3K27 methylation states are sequentially catalyzed in cycling cells

    doi: 10.64898/2026.04.30.721988

    Figure Lengend Snippet: (A) CUT&Tag signal at genes outside Polycomb domains enriched for H3K27me2 (top, n=2,268) and (B) H3K27me1 (bottom, n=3,872) ranked by replication timing (y-axis) over the S-phase fractions (x-axis). Signal is shown as fraction of the maximum (dilution) averaged across two replicates.

    Article Snippet: Confirmation of H3K27 methylation profiling with designer barcoded nucleosomes: (A) Cleavage Under Targets & Tagmentation (CUT&Tag) chromatin profiling paradigm with a synthetic nucleosome spike-in panel (EpiCypher, Cat# 19-1002).

    Techniques:

    Stable mobilization of CEBPs and PPARG upon transient TAK-981 treatment in pre-adipocytes. ( A ) ATAC-seq experimental layout in hTERT A41hWAT-SVF pre-adipocytes. Time series analysis and hierarchical clustering of significant variations in chromatin accessibility at day 0 (D0), 12 h (12h), and day (D7) after TAK-981 treatment. ( C ) GOBP analysis of ATAC-seq clusters 2 and 6 revealed in panel (B). See and for an analysis of all clusters. ( D – F ) Volcano plots displaying the results of ATAC-seq inferred differential TF activity analyses performed at D0, 12h, and D7 after TAK-981 or DMSO treatment. Colored dots indicate significant differentially mobilized TFs (TAK-981 versus DMSO). Differential binding score >0.05 and pAdj <0.001. ( G ) Time series analysis inferred TF activity over time (TAK-981 versus DMSO) at D0, 12h, and D7 after TAK-981 treatment. ( H ) Western blot analysis of CEBPB SUMOylation in DMSO and TAK-981-treated cells. ( I ) Western blot analysis of CEBPB and PPARG in DMSO, rosiglitazone, TAK-981-treated cells and cotreated hTERT A41hWAT-SVF pre-adipocytes. ( J ) Western blot analysis of PPARG in DMSO, rosiglitazone, TAK-981-treated cells and cotreated hASCs.

    Journal: Nucleic Acids Research

    Article Title: Transient SUMOylation inhibition in human pre-adipocytes stably imprints a transcriptional beiging fate

    doi: 10.1093/nar/gkag232

    Figure Lengend Snippet: Stable mobilization of CEBPs and PPARG upon transient TAK-981 treatment in pre-adipocytes. ( A ) ATAC-seq experimental layout in hTERT A41hWAT-SVF pre-adipocytes. Time series analysis and hierarchical clustering of significant variations in chromatin accessibility at day 0 (D0), 12 h (12h), and day (D7) after TAK-981 treatment. ( C ) GOBP analysis of ATAC-seq clusters 2 and 6 revealed in panel (B). See and for an analysis of all clusters. ( D – F ) Volcano plots displaying the results of ATAC-seq inferred differential TF activity analyses performed at D0, 12h, and D7 after TAK-981 or DMSO treatment. Colored dots indicate significant differentially mobilized TFs (TAK-981 versus DMSO). Differential binding score >0.05 and pAdj <0.001. ( G ) Time series analysis inferred TF activity over time (TAK-981 versus DMSO) at D0, 12h, and D7 after TAK-981 treatment. ( H ) Western blot analysis of CEBPB SUMOylation in DMSO and TAK-981-treated cells. ( I ) Western blot analysis of CEBPB and PPARG in DMSO, rosiglitazone, TAK-981-treated cells and cotreated hTERT A41hWAT-SVF pre-adipocytes. ( J ) Western blot analysis of PPARG in DMSO, rosiglitazone, TAK-981-treated cells and cotreated hASCs.

    Article Snippet: Samples for assay for transposase-accessible chromatin sequencing (ATAC-seq) were prepared based on previously published protocols with the Illumina ATAC-seq library preparation kit (20034197) [ – ].

    Techniques: Activity Assay, Binding Assay, Western Blot